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12657029, Gibco, Brazil), 1 U/mL penicillin and 1 g/mL streptomycin (#15140122, PenStrep, Gibco, USA), at 37 C, 5% CO 2 , and mycoplasm free

Cellular indices were monitored every 15 min for a total of 96 h 20

because rigorously controlled human safety data specific to the TB-500 fragment does not exist, its longer-term risk profile, including possible immune reactions, remains unknown Evidence snapshot: Evidence for TB-500 itself is preclinical, drawn mainly from cell-culture and animal studies (a 1999 rat wound-healing study and later rodent cardiac- and tendon-injury models), with a 2026 scoping review describing the musculoskeletal literature on thymosin beta-4 and TB-500 as sparse and largely preclinical