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That pre-serum wash is an easy place to add peptides rather than strip them our GHK-Cu + AHK-Cu copper peptide shampoo cleans with a gentle, aloe-based lather so the scalp is prepped, not dried out, before the serum goes on

Tests carried out on mice in order to determine LD50 as well as with the purpose of obtaining data about acute, sub chronic and chronic toxicity have been unsuccessful as this peptide applied intravenously or intraperitoneally in a broad dose range from 10 ng to 100 mg/kg of body weight, did not induce any toxic changes

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Successful transconjugants (containing a chromosomally integrated plasmid after the first homologous recombination event HR1) were selected by incubation on LB plates supplemented with kanamycin