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Timing considerations Most protocols recommend evening administration, 30-60 minutes before intended sleep

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Gene expression analysis To obtain further insight into the mechanisms of BPC-157 participation in cell signal transduction, we carried out a quantitative real-time PCR using a Signal Transduction PathwayFinder RT 2 Profiler PCR Array, which contained a set of primers for 84 key genes representative of 18 different signal transduction pathways