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DP cells will slowly overflow in DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12), 20% (v/v) fetal bovine serum (FBS) supplemented with 100 units/ml penicillin and 0.1 mg/ml streptomycin (Invitrogen, Carlsbad, CA, and Sigma, St

Although the exact mechanism of the connectivity of the gut-brain axis has not yet been elucidated, the evidence shows that gut microbiota plays an important role by producing immune factors, hormones, and metabolites that influence brain functioning