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The kynurenine pathway and excitotoxicity Kynurenines influence dopaminergic, noradrenergic, cholinergic, GABAergic, and glutamatergic neurotransmission, meaning that fluctuations in their concentrations will disrupt the neuronal-glial network and critically affect mood

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As well discuss in the elution section below, eluting GST-fusion proteins from glutathione agarose beads is gentle for most proteins compared to the harsh elution conditions required for Protein A, G, and L beads

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