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Indeed, the development of single-cell RNA sequencing analyses has led to the detection of microglial heterogeneity in both physiological and pathological conditions, suggesting that microglial activation is a dynamic response involving transcriptionally and spatially different subpopulations (Keren-Shaul et al., 2017

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Panel B of Figure 5 shows that the fluctuations in methionine input cause very large fluctuations in SAM but only modest fluctuations in intracellular methionine concentration, as suggested by the early methionine loading experiments of Finkelstein and co-workers [53, 54]