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The next day, the sections were rinsed with 0.01 M PBS, and GFAP- and CD11b-labeled sections were incubated with biotin-labeled goat anti-mouse secondary antibody (Jackson Immunoresearch, Laboratories Inc., PA, United States) diluted 1:3,000 in 2% NGS-0.1M PBS and VWF-labeled sections were incubated with biotin-labeled anti-rabbit secondary antibody (Vector Laboratories, CA, United States) diluted 1:500 in 2% NGS-0.1M PBS

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