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Detailed analysis of atpenin A5 and phenformin cytotoxicity and inhibition of metabolic activity in various cell lines As predicted from our modeling approach as tier 1 targets, atpenin A5 at 20 M and phenformin at 100 M do not show toxicity in endpoint measurements based on total cell counting when Calu-3, CaCo-2, A549, and Huh7.5 cells were exposed to the compounds for 72 h (Fig

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Additionally, due to the antioxidative activities of these compounds, they are indicated to be beneficial in protecting the body against oxidative stress [40] and neutralizing free radicals [41], as illustrated in Fig

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Rp-cAMP and H-89 were from Calbiochem of Merck Millipore (Darmstadt, Germany)

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