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Single-cell suspensions were then loaded onto microfluidic devices, and single-cell RNA sequencing (scRNA-seq) libraries were constructed according to the Singleron GEXSCOPE protocol via the GEXSCOPE Single-Cell RNA Library Kit (Singleron), which included cell disruption, mRNA trapping, cell labeling (with barcodes) and mRNA labeling (with unique molecular identifier (UMI)), reverse transcription of mRNA into complementary DNA (cDNA) and amplification, cDNA fragmentation, and finally cDNA fragment cloning

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The needle cannot reach the last drops