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Other calorie restriction interventions have noted increased alpha diversity (Shannon index) over short-term periods by implementing a high protein diet (30% of daily kcal) ( Differential abundance analysis revealed increases in several genera, including Incertae sedis and Eubacterium fissicatena group , and a decrease in Sellimonas in IF1-P
Primers (Integrated DNA technologies, Singapore) used for PCR reactions were listed in Table 1
However: It does not permanently change skin colour
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