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Cells were lysed with RIPA lysis buffer (Strong) (P0013E-1, Beyotime) and proteins denatured with 4 sodium dodecyl sulphate (SDS) loading buffer (NP0007, Invitrogen)

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Each serving delivers a standardized 125mg anthocyanin dose the same as the Cherry Juice+, not estimated by cherry count, which means nothing batch to batch

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