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This is because factors that increase the release of HDL (such as fasting, leading to low levels of HDL) increases the amount of HL bound to HDL and released into the bloodstream

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NR: n = 170 ( c ), fat mass R: n = 49

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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature

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