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After incubating, the blots were rinsed 3 times using TBST for 5 min, incubated for one hour at room temperature with the matching secondary antibody solution conjugated with horseradish peroxidase, and then rinsed with TBST once more

Furthermore, the Endocrine Societys obesity guideline recommends that patients with obesity in the absence of diabetes should discontinue weight-loss medications if safety or tolerability issues arise, and consider other therapeutic options