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Exposure to heavy metals from smoking, like cadmium and lead, may induce OS by disrupting cellular thiol buffering systems, depleting antioxidants (e.g., GSH, TXN, PRDX), and generating ROS [63, 156]

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Determination of GSH entrapment efficiency The removal of the unbound GSH from the vesicles was accomplished by ultracentrifugation at 150,000 g at 4C for 90 minutes (Ultracentrifuge Sorvall Ultra Pro 80)