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Subsequent analysis of morphine-induced constipation in VgluT2-Cre mice injected with AAV-DIO-shMOR-EGFP revealed that PVN Glu -specific MOR knockdown resulted in significantly higher fecal output and fecal water contents, as well as significantly higher transit rates and motility in small intestine of morphine-treated mice compared to that of corresponding AAV-DIO-EGFP control animals (Fig