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The macrophages uptake lipids and lipoproteins and secrete cytokines and chemokines to further recruit CD4 T cells and smooth myocytes in the intima layer of vessels

Their structural similarity (both seven-amino-acid stabilized fragments) is part of why they're often grouped together, but their research applications are genuinely distinct

The thermal cycling conditions consisted of the following: cDNA synthesis for 10 minutes at 50 C, initial denaturation and Taq Polymerase activation for 5 minutes at 95 C, followed by 40 cycles of denaturation at 95 C for 10 seconds and annealing/extension at 60 C for 30 seconds

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