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The antibodies against phospho-AKT(Ser473) (4060), AKT(pan) (4691), phospho-p70S6K(Thr389) (9205), p70S6K (2708), phospho-ERK1/2(Thr202/Tyr204) (4370), ERK1/2 (4695), phospho-S6 ribosomal protein (Ser235/236) (4858), S6 ribosomal protein (2217), phospho-MDM2(Ser166) (3521), phospho-MEK1/2(Ser217/221) (9154), MEK1/2 (8727), MEK1 (12671), and cleaved caspase 3 (9664) were purchased from Cell Signaling (1:1000 or 1:2000 dilution)
Given the dendritic impairments induced by Rot, we next evaluated synaptic integrity