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The non-neuronal cholinergic system (NNCS) is made up of neurotransmitter acetylcholine, its synthesizing and degrading enzymes, transporters, and receptors within epithelial cells in airways, intestine, skin, urothelium, vagina, placenta, cornea, granulosa cells, endothelial cells, immune cells and mesenchymal cells ( In this review, several immune cells that express components of NNCS and respond to neurotransmitters, specific agonists, and antagonists and their contribution to inflammation and autoimmunity are discussed