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As a control to check for artifacts, a single negative control (PCR with nuclease-free water as template DNA) as well as a positive control using a mock community (ZymoBIOMICS, number D6300) were included throughout each sequencing run

You should also get medical advice for heavy or lasting vomiting or diarrhea, or for a new lump or swelling in the neck, hoarseness, or difficulty swallowing