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Low concentrations of testosterone had no effects on cell viability , whereas at high concentrations the cell viability decreased with incremental increases in hormone concentration. The testosterone-induced apoptosis described in this study occurs through overactivation of intracellular Ca2+ signaling pathways

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After pre-incubation, they were transferred to a 24-well plate supplemented with a fresh medium and topically exposed to 30 l of negative control (PBS), positive control (5% SDS causes keratinocyte death), and MB from 0.2 M to 500 M for 60 min at room temperature

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