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Effect of NNMT inhibitor on adipocyte differentiation quantitated with oil red O staining 3T3-L1 cells were cultured in 60 mm diameter dishes (8.410 4 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)

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(2) M A Heffernan 1, A W Thorburn, B Fam, R Summers, et al (2001) Increase of fat oxidation and weight loss in obese mice caused by chronic treatment with human growth hormone or a modified C-terminal fragment International Journal of Obesity and Related Metabolic Disorders, 2001 Oct, Volume 25 (Issue 10), Pages 1442-9