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Postfixative was removed and cells were washed (five times for 1 min each) with 0.1 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and then washed (five times for 1 min each) with cold double-distilled H 2 O (ddH 2 O) on ice

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In this process, GSH is constantly oxidized to GSSG by the enzyme glutathione peroxidase.To maintain the intracellular redox balance, GSH is replenished through the reduction GSSG by glutathione reductase enzyme

Carlo Borromeo, Milano)