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Brief report: a deficiency of carnitine-acylcarnitine translocase in the inner mitochondrial membrane

doi: 10.2147/ciia.2006.1.4.307

Immunoblotting Protein samples were separated by SDS-PAGE, transferred to PVDF membrane, and probed with following antibodies: HNF4 (Santa Cruz, sc-374229, clone H-1, Lot B2417, 1:500), BHMT (Santa Cruz, sc-390299, clone H-7, Lot B1913, 1:500), CBS (Santa Cruz, sc-133154, clone B-4, Lot D0918, 1:1000), CDO1 (ThermoFisher Scientific, PA5-38005, Lot UD2757664A, 1:1000), MAT1A (Abcam, ab129176, Lot GR91375-9, 1:1000), CTH (Proteintech Group, 12217-1-AP, Lot 00076987, 1:1000), Puromycin (Millipore Sigma, MABE343, clone 12D10, Lot 3379285, 1:1000), Actin (Millipore Sigma, MAB1501, clone C4, Lot 3132961, 1:10,000), HRP-conjugated alpha Tubulin (Proteintech, HPR-66031, mouse monoclonal, Lot 21000018, 1:2000)

the Met + Pio group ( n = 75) received metformin plus pioglitazone (500 mg, 15 mg, respectively) and placebo (citric acid plus calcium carbonate), twice daily for 12 weeks