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RNA extraction and RT-qPCR Total RNA was extracted using the GeneJET RNA Purification Kit (K0732, Thermo) according to the manufacturers instructions

Combination therapy with acarbose and metformin has been demonstrated, in multiple studies, to be more effective for the control of blood glucose than treatment with either drug alone (Mooradian 1999

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A placebo-controlled study on the use of low-dose naltrexone (4.5 mg per day at bedtime) suggested that the drug could resolve mucosal inflammation and induce clinical remission in patients with moderate-to-severe Crohns disease (Smith 2011)