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A MiniTrap desalting column was used to change the buffer of the protein samples to 20 mM Na 2 HPO 4 , p H 7.5, 50 mM NaF, 5% ( w / v ) glycerol containing either 1 mM DTT or 0.5 mM GSH as reducing agents

There is discussion in the literature suggesting GHK Cu may influence gene expression in ways that resemble more youthful patterns, at least in certain experimental models

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Thus, the formation of the dehydro-alanine glutathione species (that observed at m / z 272 in the spectra of glutathione-bound metals) is a key to the possible explanation of the ability of metals such as lead and cadmium to generate oxidative stress in cells without the necessity that the electrochemically silent metal itself change its oxidation state