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This dye exhibits two distinct emission peaks: red fluorescence from J-aggregates, indicative of hyperpolarized mitochondria (high membrane potential), and green fluorescence from JC-1 monomers, associated with depolarized mitochondria (low membrane potential)

Common mistakes include: Injecting into muscle instead of fatty tissue Injecting through clothing Rushing the injection and removing the pen too early Using the same injection site repeatedly Not allowing alcohol to dry before injecting Avoiding these errors reduces pain, bruising, and inconsistent absorption

They should not be used as replacements for prescribed psychiatric medications without medical supervision