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Bound proteins were eluted with His elution buffer (50 mM Tris-HCl pH 7.5, 50 mM NaCl, 250 mM imidazole, 2.5 mM ME)

Moreover, GLP-1RAs are known to reduce lipotoxicity and insulin resistance in the liver and improve metabolic dysfunction, which are the key pathogenetic factors of NAFLD

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