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10.1016/j.jneuroim.2007.01.003 180

Enzyme expression was induced by the addition of 100 M isopropyl -d-1-thiogalactopyranoside, the cultures were incubated for another 3.5 h at 30 C, 140 rpm using a 5 cm shaking diameter, and the final OD 600 (optical density at 600 nm) was determined using a CO8000 cell density meter (WPA biowave)

Likewise, the counteraction includes the myocardial lesions after 2 isoprenaline applications (i.e., re-infarction), if the next application for re-infarct induction occurs at the point of 24 h and assessment after the end of the subsequent 24 h period

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