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RNA density was measured using NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA)

Briefly, 10 L of CCK-8 reagent was added to the 100 L cell suspension and incubated for 3 h at 37C

215 reported that chemically altered collagen membranes were released by the addition of bFGF and keratinocyte growth factor (KGF) which facilitated cellular proliferation, faster cell migration and tissue regeneration

Most notably, acute colitis induced by the administration of DSS in these mice failed to resolve even when DSS was removed from the drinking water, and this was found in contrast to the complete recovery seen in WT littermate mice