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Triglycerides levels were determined from the supernatants using the Triglyceride Liquid Assay (Sentinel Diagnostics) following manufacturers instructions as in ( 2.6 Gene expression RNA was extracted from frozen liver tissues using the RNeasy mini kit (Qiagen), treated with DNase I (Sigma-Aldrich), then reverse-transcribed with M-MLV reverse transcriptase (Promega)

Together these results suggested that the balancing of oxidative cellular status involves the increase in active GSH efflux to extracellular medium [36]