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Sections were dewaxed and stained with a rabbit polyclonal anti-FITC (711900, Thermo Fisher Scientific, 1/100) plus secondary Alexa Fluor 488 antibody (Life Technologies, 1/200), and guinea pig anti-human insulin (A0564, Dako, 1/1000) plus secondary Alexa Fluor 546 antibody (Life Technologies, 1/1000) prior to confocal imaging as above

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UBAC2 binds to gp78 and ubiquitin regulatory X (UBX) domain-containing protein 8 (UBXD8), contributing to the formation of a dislocation channel