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As SPHKAP has previously been shown to bind to the mitochondrial cristae junction regulator and MICOS complex component MIC19 40 , a prominent PKA substrate involved in both cristae assembly during mitochondrial remodelling 43 and in regulation of ERMCSs, we next tested whether exendin-4 stimulation would trigger MIC19 phosphorylation in WT INS-1 832/3 cells using a MIC19 immunoprecipitation strategy coupled with western blotting with an antibody against PKA phosphosites (RRxpS/T) 44

Pharmacology, physiology, and mechanisms of action of dipeptidyl peptidase-4 inhibitors