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TNF- activates multiple signaling pathways through binding to its receptors (TNFR1 and TNFR2), inducing the release of inflammatory mediators, cell proliferation, apoptosis, and immune cell recruitment, ultimately leading to chronic intestinal inflammation

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In diet-induced obese (DIO) mouse models, NNMT inhibition with 5-Amino-1MQ was associated with reduced white adipose tissue mass without affecting food intake, suggesting changes in energy expenditure rather than appetite suppression

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