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The amount of unentrapped (free) drug in the supernatant was quantified by HPLC, and EE was calculated using the following equation: High-resolution Transmission Electron Microscopy (TEM) The SLN dispersion was diluted 1:100 and stained with 1% phosphotungstic acid

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This reaction is catalysed by the enzyme folate conjugase on the brush border of enterocytes in the proximal small intestine 887,888

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