The diluent is added slowly against the inside wall of the vial rather than directly onto the powder, and the vial is swirled gently rather than shaken vigorously, because mechanical agitation can stress peptide structure and generate foaming
Curr Opin Chem Biol 28:174180 Dubowchik GM, Firestone RA, Padilla L, Willner D, Hofstead SJ, Mosure K, Knipe JO, Lasch SJ, Trail PA (2002) Cathepsin B-labile dipeptide linkers for lysosomal release of doxorubicin from internalizing immunoconjugates: model studies of enzymatic drug release and antigen-specific in vitro anticancer activity
The harmonic restraints were released in five steps, transitioning the system from an NVT to an NPT ensemble over 100 ps
What is the difference between research-grade and cosmetic-grade GHK-Cu for injection