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Thus, by promoting intimal hyperplasia, these vascular disorders (which are related to smooth muscle cell proliferation, collagen accumulation, and the extracellular matrix modification that may result from homocysteinylation of the vascular wall structure leading to connective tissue deterioration) might contribute to the atherogenic tendency of hyperhomocysteinemia

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All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction

As the disease progressed, interestingly, a striking downregulation was observed in miR-21 transcript levels