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Figure 4: Photograph of Professor Georg L Zuelzer, unknown photographer and date, Historical Archive, Humboldt University, Berlin

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For retrograde monosynaptic tracing of the PVN DMV circuit, the helper viruses containing rAAV-EF1-DIO-RVG-WPRE-hGH pA (AAV-DIO-RVG, AAV2/9, 4.59 10 12 vg/mL) and rAAV-EF1-DIO-H2B-mCherry-T2A-TVA-WPRE-hGH pA (AAV-DIO-TVA-mCherry, AAV2/9, 5.56 10 12 vg/mL) (1:2, 150 nL) were injected into the DMV, and scAAV2/Retro-hSyn-Cre-WPRE (20 L) was injected into the small intestinal wall of C57 mice