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In addition, Serine and arginine rich splicing factor 9 (SFRS9) can upregulate the expression of GPX4 by binding to GPX4 mRNA, which promote the growth of CRC, while SFRS9 knockdown significantly inhibited tumor growth in nude mice (Wang R

Immunofluorescence microscopy Cells (HEK293T, U2OS, or HeLa) grown in glass-bottom dishes (ibidi) were fixed using 4% formaldehyde (Polysciences) and permeabilized using 0.5% Triton X-100 (Sigma-Aldrich)