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In vitro glucose-stimulated insulin secretion (GSIS) Insulin secretion was measured using the Krebs Ringer Bicarbonate (KRB) buffer (119 mM NaCl, 5 mM KCl, 2.5 mM CaCl 2 , 1.2 mM KH2PO 4 , 1.2 mM MgCl 2 , 10 mM HEPES, 25 mM NaHCO 3 , and 0.2% BSA)