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Description

AGPAT3 promoted RSL3 induced lipid peroxidation and ferroptosis in the ER+ cell lines T47D and MCF-7, although was dispensable in the TNBC Cal51 cells line that have higher levels of ACSL4

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This section provides everything needed to understand and implement this popular protocol

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The fluorescence was analyzed by flow cytometry (Attune NxT, ThermoFisher Scientific)

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This restoration of metabolic cofactor availability enables enhanced function of NAD+-dependent enzymes including sirtuins, poly(ADP-ribose) polymerases, and various deacetylases that regulate cellular metabolism, stress responses, and aging processes

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