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Metab Brain Dis 24(1):103117

Following this, the likely role of APP and A isoforms are as copper scavengers, since they both contain copper binding domains with high affinity for copper ions (Kong et al., 2008

Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set the counter to zero with distilled Preheat Reagent II in water bath for 30 minutes: 37 (mammal cell) or 25 (other species)