2.3 Preparation of sperm suspensions On February 24 th , 2024, frogs were euthanised according to methods approved by the University of Wollongong s Animal Ethics Committee (see section 2.8 Ethics review statement below), and testis tissue immediately extracted, weighed, and macerated in 170L of simplified amphibian ringer (SAR: 113mM NaCl, 1mM CaCl 2 , 2mM KCl, 3.6mM NaHCO 3 )
To find the dose per unit, you just divide the total peptide amount by the total units: 5000mcg / 200 units = 25mcg per unit
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