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Both male and female mice were housed under controlled conditions of 12 h:12 h light-dark cycles and at 22 C temperature and 4555% humidity and fed ad libitum a standard rodent chow diet (CHD) or high fat diet (HFD) (60% of calories from fat) for 12 (males) or 24 (females) weeks starting at week 8 of age

There are few Pro specific proteases and these include DPP4 and FAP (the type II integral membrane proteins), intracellular DPPII/DPP7 that localizes to the vesicular system, soluble and cytosolic DPP8 and DPP9, and intracellular prolyl oligopeptidase (POP)

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2,46 Long-term follow-up studies have indicated an elevated fracture risk associated with CR