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Cells were washed with PBS and fixed with 4% paraformaldehyde (PFA) for 10 minutes at 4C, permeabilised for 15 seconds in a liquid nitrogen bath, blocked in 1% BSA / PBS for 1 hour at room temperature, incubated with D4H*-mCherry (1:25 in 1% BSA / PBS) for 2 hours, washed with PBS and post-fixed in 4% PFA for 10 minutes at room temperature

Broader Cancer Outcomes and Mechanistic Insights For most other cancers, recent clinical evidence demonstrates largely positive outcomes, with several meta-analyses showing no excess risk and even a reduced risk for hepatocellular carcinoma and colorectal cancer (relative to insulin users)

At the lowest dose tested (1 g kg 1 ), P5 significantly decreased fasting blood glucose levels and significantly attenuated glucose intolerance whereas an equivalent dose of Ex4 had no effect (Fig

2010;35(5)(Diabetes suppl):8-13