When we find out more about how these metabolic pathways mix with each other, it will help us make better plans to treat obesity
In good health, Megan Pfeffer, BHSc, CPN Clinical Nutritionist | Founder, I Choose Health Board Member, Lipedema World Alliance Advisory Board Member, Lipoedema Australia References Aneke-Nash, C., Hung, K
Naselli-Flores L, Zohary T, k P
Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations