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Acknowledgements This work was supported in part by the Cardiovascular Diabetology Research Foundation (RA 58-040-684-1), Holon, Israel

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Maintain adequate calcium (1,000-1,200 mg daily) and vitamin D intake, consume sufficient protein (1.0-1.2 g/kg body weight), perform regular weight-bearing and resistance exercise, and discuss appropriate monitoring with your healthcare provider based on your individual risk factors

The choice of solvent, concentration, and handling procedures are determined by the specific requirements of each research protocol and analytical method