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All samples were serially diluted in assay buffer in duplicate and incubated with cells for 30 min at RT before detection with the cAMP dynamic 2 HTRF assay (Cisbio assays, Bedford, MA) as described previously 51

If phase 3 trials confirm its promise, clinicians in the second half of this decade may have access to a tool that not only rivals surgery for weight loss, but also transforms the prognosis of diseases like metabolic liver disease and type 2 diabetes