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After one week of recovery period, only the recipient mice that exceeded the initial body weight (i.e., body weight of the recipient plus weight of the transplant) were subjected to a HFD (D12451) (45.0 % kcal from fat) (Research Diets Inc.) for 8 weeks

As time passes, the hormone breaks down, which lowers its effectiveness

The compound was first reported in Al-Obeidi, Castrucci, Hadley, and Hruby, J Med Chem 32 (12):255561 (1989) (PMID 2555512), building on the earlier open-chain superpotent precursor [Nle, D-Phe]--MSH reported by Sawyer, Sanfilippo, Hruby, Engel, Heward, Burnett, and Hadley in PNAS 77 (10):57548 (1980) (PMID 6777774)

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