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Target sequence was selected by CRISPR-PLANT ( All the vectors were transformed into Agrobacterium tumefaciens strain GV3101 using electroporation

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Its key mechanism lies in the activation of AMP-activated protein kinase (AMPK) , a cellular energy sensor often referred to as the bodys metabolic master switch. When AMPK is activated by MOTS-c, several critical processes occur: Improved Glucose Utilization MOTS-c enhances insulin sensitivity, enabling cells to absorb and use glucose more effectively