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Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 45 mice per genotype) from 46-week-old mice was digested in 1 mg ml 1 collagenase D (Roche) with 1.5% BSA in sterile Hanks Balanced Salt Solution and incubated in a 37 C shaking water bath for 1 h

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AP and PR performed quantification of cytokines corrected the manuscript

Data availability Original data generated and analyzed during this study are included in this published article, the supplementary material, or are available from the corresponding authors on reasonable request